Research Articles
EPI Activity Spectrum Across RND Pump Homologs: Decoding Efflux Inhibition for Next-Generation Antimicrobials
This article provides a comprehensive analysis of the activity spectrum of Efflux Pump Inhibitors (EPIs) across homologs of the Resistance-Nodulation-Division (RND) superfamily of multidrug efflux pumps.
Unveiling Resistance: Advanced Methods for Detecting Antibiotic Resistance Gene Cassettes in Clinical and Environmental Samples
This article provides a comprehensive review of state-of-the-art detection methods for antibiotic resistance gene cassettes (ARG-cassettes), critical mobile genetic elements driving the global antimicrobial resistance (AMR) crisis.
DISCERN Tool for LLMs: A Framework for Evaluating Antibiotic Advice Quality in Biomedical Research
This article provides a comprehensive analysis of the DISCERN instrument as an evaluation framework for the quality of antibiotic advice generated by Large Language Models (LLMs).
Strategies for Controlling Integron-Mediated Gene Cassette Shuffling: A Research and Development Guide
This article provides a comprehensive overview for researchers, scientists, and drug development professionals seeking to understand and manipulate integron-mediated gene cassette shuffling, a key mechanism of horizontal gene transfer driving...
Horizontal Gene Transfer Mechanisms: How Conjugation, Transduction, and Transformation Drive Antibiotic Resistance in Bacteria
This article provides a comprehensive analysis of the three primary horizontal gene transfer (HGT) mechanisms—conjugation, transduction, and transformation—and their critical role in the dissemination of antibiotic resistance genes (ARGs).
Natural Competence in Priority Pathogens: Mechanisms, Methods, and Medical Implications for AMR and Virulence
This comprehensive review analyzes the phenomenon of natural competence—the ability of bacteria to actively take up extracellular DNA—in globally significant priority pathogens.
Efflux Pump Superfamilies: A Comparative Analysis of Mechanisms and Therapeutic Targets in MDR Pathogens
This article provides a comprehensive analysis of the major efflux pump superfamilies—RND, MFS, MATE, SMR, and ABC—that drive multidrug resistance (MDR) in critical Gram-negative and Gram-positive pathogens.
Clinical vs. Environmental Integrons: A Comparative Genomic Analysis of Allelic Diversity, AMR Cassettes, and Transmission Risk
This article provides a comprehensive comparative analysis of integron alleles and gene cassette arrays from clinical and environmental reservoirs.
AI Prescribing Showdown: Benchmarking ChatGPT-o1 vs Claude 3.5 Sonnet for Antibiotic Accuracy in Clinical Scenarios
This article presents a comparative evaluation of the latest generative AI models, OpenAI's ChatGPT-o1 and Anthropic's Claude 3.5 Sonnet, in the critical domain of antibiotic prescribing accuracy.
Beyond the Vector Copy Number: A Comprehensive Guide to Measuring and Optimizing Gene Therapy Transduction Efficiency
This article provides a critical examination of the current landscape and persistent challenges in quantifying transduction efficiency for gene and cell therapies.