Research Articles
Targeting Horizontal Gene Transfer: A Comparative Analysis of CRISPR-Based, Phage-Derived, and Small-Molecule Inhibition Strategies in Combating Antibiotic Resistance
This article provides a comprehensive comparison of modern strategies to inhibit Horizontal Gene Transfer (HGT), a primary driver of multi-drug resistant bacterial infections.
Biochar vs. SCFAs: Comparative Strategies to Curb Antibiotic Resistance Gene Spread in Biomedical Applications
This article provides a comprehensive analysis of two promising strategies for mitigating the environmental and clinical spread of Antibiotic Resistance Genes (ARGs): biochar and Short-Chain Fatty Acids (SCFAs).
EUCAST Broth Microdilution: The Definitive Guide for Antimicrobial Susceptibility Testing in Research & Development
This comprehensive guide details the European Committee on Antimicrobial Susceptibility Testing (EUCAST) broth microdilution (BMD) method, the gold standard for in vitro antimicrobial susceptibility testing (AST).
Defeating the Unbeatable: ESKAPE Pathogens, Biofilm-Mediated Treatment Failures, and the Race for Novel Therapeutics
This comprehensive review addresses the critical challenge of antimicrobial resistance posed by ESKAPE pathogens (*Enterococcus faecium*, *Staphylococcus aureus*, *Klebsiella pneumoniae*, *Acinetobacter baumannii*, *Pseudomonas aeruginosa*, and *Enterobacter* species) through the lens...
EPI Activity Spectrum Across RND Pump Homologs: Decoding Efflux Inhibition for Next-Generation Antimicrobials
This article provides a comprehensive analysis of the activity spectrum of Efflux Pump Inhibitors (EPIs) across homologs of the Resistance-Nodulation-Division (RND) superfamily of multidrug efflux pumps.
Unveiling Resistance: Advanced Methods for Detecting Antibiotic Resistance Gene Cassettes in Clinical and Environmental Samples
This article provides a comprehensive review of state-of-the-art detection methods for antibiotic resistance gene cassettes (ARG-cassettes), critical mobile genetic elements driving the global antimicrobial resistance (AMR) crisis.
DISCERN Tool for LLMs: A Framework for Evaluating Antibiotic Advice Quality in Biomedical Research
This article provides a comprehensive analysis of the DISCERN instrument as an evaluation framework for the quality of antibiotic advice generated by Large Language Models (LLMs).
Strategies for Controlling Integron-Mediated Gene Cassette Shuffling: A Research and Development Guide
This article provides a comprehensive overview for researchers, scientists, and drug development professionals seeking to understand and manipulate integron-mediated gene cassette shuffling, a key mechanism of horizontal gene transfer driving...
Horizontal Gene Transfer Mechanisms: How Conjugation, Transduction, and Transformation Drive Antibiotic Resistance in Bacteria
This article provides a comprehensive analysis of the three primary horizontal gene transfer (HGT) mechanisms—conjugation, transduction, and transformation—and their critical role in the dissemination of antibiotic resistance genes (ARGs).
Natural Competence in Priority Pathogens: Mechanisms, Methods, and Medical Implications for AMR and Virulence
This comprehensive review analyzes the phenomenon of natural competence—the ability of bacteria to actively take up extracellular DNA—in globally significant priority pathogens.